Comparison of immunoreactive renin and plasma renin activity in human plasma.

Sample requirements for plasma renin activity and immunoreactive renin.

Measurement of renin activity in human plasma.

• Many methods for measurement of renin activity have been described since Tigerstedt and Bergman first made an extract of kidney and showed that when injected it raised blood pressure. Those methods which rely on the rise of blood pressure following such injections suffer from three main disadvantages. First, the magnitude of response to a given dose may vary and tachyphylaxis can be elicited....

Direct radioimmunoassay of human renin: comparison with renin activity in plasma and amniotic fluid.

Human plasma and amniotic liquid were activated by dialysis at pH 3.3. Then, renin before and after acidification was determined by two methods: enzymatic activity measurement, and direct radioimmunoassay. The identity between nonactivated and activated renin in plasma and amniotic fluid on the one hand, and pure renin on the other, was demonstrated by the dilution curves in radioimmunoassay. A...

Plasma renin activity and plasma prorenin assays.

Sensitivity and accuracy are essential features of an assay of plasma renin activity (PRA) because the normal concentration of PRA is only 1 pmol/L, and subnormal concentrations have diagnostic relevance. Conditions for blood collection need to be standardized but the conditions are not difficult for outpatients. For routine diagnostic purposes blood should be collected from ambulatory (ideally...

Determinants of Plasma Renin Activity

The plasma renin activity (PRA) is affected by a number of environmental factors. However, significant heritability has been shown for the activity. A hypothesis that a candidate regulatory single-nucleotide polymorphism, C-5312T, of human renin gene should have a significant effect on PRA was elucidated and updating of independent determinants of PRA was attempted. Cross sectional study.